Abstract
Two-year-old seedlings of European mountain ash (Sorbus aucuparia L.) were differentially exposed to freezing temperatures. Basal portions of plants were maintained above 0 °C with electrical heating cables and insulation during a rapid drop in ambient air temperature from 5 to −30 °C. Plants were inoculated at three heights on the stem with a nonaggressive stem canker pathogen, Botryosphaeria dothidea, to determine the predisposing effect of differential freezing on the susceptibility of whole plant stems. Exposed portions of stems were predisposed to attack by B. dothidea but unfrozen portions remained resistant. The pathogen colonized frozen tissue near the margins of insulation but did not colonize unfrozen portions. This method provided an interface between resistant and susceptible tissue on intact whole plants.
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