Abstract
As a chemiluminescence (CL) probe, 3,7-dihydro-6-{4-{2-( N′-(5-fluoresceinyl) thioureido)ethoxy}phenyl}-2-methylimidazo{1,2-a}pyrazin-3-one dosium salt (FCLA) can sensitively and specifically react with singlet oxygen ( 1O 2) and superoxide (O 2 −). Based on the fact that photosensitization action involves 1O 2 generation, this report presents a novel method for localization of tumor mediated by FCLA. In experiments, FCLA was used as an optical reporter of 1O 2 produced from photosensitization reaction of hematoporphyrin derivative. The effects of azide inhibition and deuterium enhancement on photosensitized CL indicated that 1O 2 molecules generated during photosensitization action could be measured and imaged. In vivo experiments in vivo with tumor-bearing nude mice showed a clear CL image of tumor. The study suggests that CL imaging method may be applicable to the detection of 1O 2 and the diagnosis of superficial tumors.
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