Abstract
Localization of the R-membrane in eye cup preparations and in isolated retina preparations of the bullfrog has been made by an intracellular marking technique. The marking was made when the microelectrode was located “inside the R-membrane”, as characterized by a resting potential of 30–60 mV and by a slow rounding of the potential drop against rectangular current pulses passed radially across the retina. In the eye cup marks were found in the pigment epithelium cells, but in the isolated retina they were found inside single receptors or in the receptor layer, thus revealing a dual nature of the R-membrane. Earlier views concerning the localization of the R-membrane are briefly discussed in the light of these results.
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