Abstract
Health of the retinal pigment epithelium (RPE) beneath the retina and photoreceptors in the eye is important for the vision. Human embryonic stem cells can be used to produce RPE cells and cultured tissue transplants could be used to replace the damaged RPE tissue. In this paper we describe our work-in-progress and introduce a method how to verify if the grafting of the RPE tissue beneath the retina has succeeded. The method is based on animal model in vitro electroretinography (ERG). Reference ERG is measured from the eyes of healthy mice and compared to measurements made with the eyes of mice that have grafted RPE tissue. ERG is measured with micro electrode array (MEA) from an eye cup preparation. Healthy eye cup has typical wave forms in ERG signal and successful grafting is verified by showing the same wave forms in reference measurements and in measurements made with the eyes with grafted RPE tissue. We have made test ERG measurements with the MEA system and proven that it works correctly. There is work in progress in order to produce reliable reference signals for eye cup preparations and for the assessment of the grafts.
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