Abstract
Background/Aims: Histamine H<sub>2</sub> receptor antagonists are widely used for the treatment of peptic ulcer disorders. However, whether the H<sub>2</sub> receptor is present in parietal or immune cells in the lamina propria remains controversial. This study is designed to determine the H<sub>2</sub> receptor localization immunohistochemically using an antibody against the newly cloned mouse histamine H<sub>2</sub> receptor. Methods: We cloned the mouse histamine H<sub>2</sub> receptor gene and generated a specific antipeptide antibody against the C terminus. Immunohistochemical studies were performed with this antibody and with a monoclonal antibody against H<sup>+</sup>/K<sup>+</sup> adenosine triphosphatase (ATPase). Results: Histamine H<sub>2</sub> receptors were localized on the plasma membrane and on the cytoplasm just beneath the plasma membrane on the basolateral sides of gastric cells. Confocal microscopy of double-stained sections using the monoclonal antibody against H<sup>+</sup>/K<sup>+</sup> ATPase, a specific parietal cell marker, showed that histamine H<sub>2</sub> receptors colocalized with H<sup>+</sup>/K<sup>+</sup> ATPase. No specific histamine H<sub>2</sub> receptor immunoreactivities were observed in the submucosal regions. Conclusion: The H<sub>2</sub> receptor is localized in the gastric parietal cell.
Published Version
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