Abstract
Reduced nicotinamide adenine dinucleotide (NADH) oxidase activity (EC 1.6.99.1) was assessed in membrane and supernatant fractions obtained by osmotic lysis and differential centrifugation from Acholeplasma granularum, A. modicum, A. axanthum, and Mycoplasma strain 14. NADH:ferricyanide oxidoreductase activity (EC 1.6.99.1) was also assessed in fractions of A. laidlawii B and the mycoplasma. NADH oxidase and NADH:ferricyanide oxidoreductase activities were localized in the membrane fraction of the acholeplasmas and in the supernatant fraction of the mycoplasma. The localization of NADH oxidase in membrane fractions of cells of Acholeplasma and supernatant fractions of cells of Mycoplasma after osmotic lysis is useful in differentiating these genera.
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