Abstract
Previous studies have suggested that in the rat, small intestine is the source for rise in plasma histaminase levels seen after heparin administration. The cellular location of histaminase in intestine and the mechanism of heparin release have not been previously investigated. The present study identifies intestinal villus cells rather than crypt cells as the location of intestinal histaminase; at this site, the enzyme is not associated with brush border. Heparin added to incubations containing isolated intestinal cells did not release histaminase into the medium. Perfusion of intestinal vasculature with heparin caused a prompt release of this enzyme into venous effluent. The present investigation. therefore, suggests that heparin releases histaminase from vascular binding sites rather than directly from parenchymal cells. The use of isolated intestine with perfusion of the vasculature could serve as a useful tool for further defining the relationship between the sites of synthesis and the binding sites involved with heparin releasable enzymes such as histaminase.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
