Abstract
The localization of gonadotropinreleasing hormone (Gn-RH) in the hypothalamus of male and female mice was investigated. Serial paraffin sections were prepared. Rabbit antiserum to synthetic Gn-RH was used as the first reactant in the immunoperoxidase bridge technique. Final reaction products with 3,3' diaminobenzidine were seen by light microscopy. Addition of Gn-RH to the first antibody blocked the staining of immunoreactive tissue Gn-RH. In the hypothalamus of both sexes, the immunoperoxidase technique localized Gn-RH in the perikarya of some arcuate neurons, and throughout the course of numerous tanycytes of the median eminence. Gn-RH staining was also found associated with the organum vasculosum of the lamina terminalis in one of the brains. The releasing hormone was found neither in axons of arcuate neurons nor in other neurons of the hypothalamus. These results suggest that Gn-RH may be synthesized in arcuate neurons, and that tanycytes may provide a major pathway for the secretion of this releasing...
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