Local anesthetic, anti-inflammatory and analgesic activities of nanoparticles green-formulated by plant extract

Abstract

• The results revealed a spherical morphology in the range size of 10–50 nm for AuNPs. • The IC50 of AuNPs and BHT against DPPH were 18 and 12 µg/mL, respectively. • It seems the recent nanoparticle may be use as a novel therapeutic drug in future. In the current research, we decided to formulate gold nanoparticles in aqueous medium using plant leaf aqueous extract as a new therapeutic drug for determining the local anesthetic, anti-inflammatory and analgesic activities in the in vivo condition. The common chemical tests i.e., UV–Visible Spectroscopy (UV–Vis), Transmission Electron Microscopy (TEM), and Fourier Transformed Infrared Spectroscopy (FT‐IR) were used for characterizing of nanoparticles. To survey the cytotoxicity properties of nanoparticles, MTT assay was used on the human normal cell lines. Analgesic activity was tested in mice using various doses orally. Acetic acid-induced writhing episodes were significantly and dose-dependently reduced. The local anesthetic activity were tested in frog and guinea pig models, and it was seen that in both animals, the nanoparticle produces significant local anesthetic activity. At the same dose, its anti-inflammatory activity was also tested. Carrageenin-induced paw edema in mice was significantly reduced after oral administration. It seems the recent nanoparticle may be use as a novel therapeutic drug with local anesthetic, anti-inflammatory and analgesic activities in future.