LncRNA-ZFAS1 promotes the progression of endometrial carcinoma by targeting miR-34b to regulate VEGFA expression.

Hongli Zhu,Qihui Cheng,Hong Cai

doi:10.1515/med-2021-0362

Hongli Zhu, Qihui Cheng + Show 1 more

Open Access

https://doi.org/10.1515/med-2021-0362

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Abstract

Zinc finger nuclear transcription factor, X-box binding 1-type containing 1 antisense RNA 1 (ZFAS1) functions as an oncogenic long noncoding RNA (lncRNA) to promote proliferation and metastasis of endometrial carcinoma cell; however, the underlying mechanism has not been fully understood. First, RT-qPCR analysis of endometrial carcinoma tissues and cells showed that ZFAS1 was enriched in endometrial carcinoma tissues and cells. miR-34b was reduced in endometrial carcinoma and suggested negative correlation with ZFAS1 in endometrial carcinoma. Second, functional assays demonstrated that siRNA-mediated silence of ZFAS1 suppressed endometrial carcinoma cell proliferation and metastasis. Third, ZFAS1 bind to miR-34b and negatively regulate expression of miR-34b in endometrial carcinoma cells. miR-34b also bind to and negatively regulate expression of vascular endothelial growth factor A (VEGFA) in endometrial carcinoma cells. Lastly, knockdown of miR-34b counteracted with the suppressive effects of ZFAS1 silence on endometrial carcinoma cell proliferation and metastasis. In conclusion, lncRNA ZFAS1 functioned as an oncogene to promote endometrial carcinoma cell proliferation and metastasis through miR-34b/VEGFA axis.

Highlights

Zinc finger nuclear transcription factor, X-box binding 1-type containing 1 antisense RNA 1 (ZFAS1) functions as an oncogenic long noncoding RNA to promote proliferation and metastasis of endometrial carcinoma cell; the underlying mechanism has not been fully understood
Loss-of-functional assays were applied to detect the role of ZFAS1 on endometrial carcinoma progression. siRNAs-mediated knockdown of ZFAS1 was verified by qRT-PCR (Figure 2a). si-ZFAS1#2 showed lower expression of ZFAS1 than si-ZFAS1#1 (Figure 2a)
Previous study has shown that upregulation of ZFAS1 in endometrial carcinoma predicted poor prognosis of the patients, and in vitro loss-of-functional assays validated the oncogenic role of ZFAS1 on endometrial carcinoma [13]

Summary

Introduction

Abstract: Zinc finger nuclear transcription factor, X-box binding 1-type containing 1 antisense RNA 1 (ZFAS1) functions as an oncogenic long noncoding RNA (lncRNA) to promote proliferation and metastasis of endometrial carcinoma cell; the underlying mechanism has not been fully understood. Functional assays demonstrated that siRNAmediated silence of ZFAS1 suppressed endometrial carcinoma cell proliferation and metastasis. ZFAS1 bind to miR-34b and negatively regulate expression of miR-34b in endometrial carcinoma cells. MiR-34b bind to and negatively regulate expression of vascular endothelial growth factor A (VEGFA) in endometrial carcinoma cells. Knockdown of miR-34b counteracted with the suppressive effects of ZFAS1 silence on endometrial carcinoma cell proliferation and metastasis. LncRNA ZFAS1 functioned as an oncogene to promote endometrial carcinoma cell proliferation and metastasis through miR34b/VEGFA axis

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Conclusion