LncRNAs Participate in Post-Resuscitation Myocardial Dysfunction Through the PI3K/Akt Signaling Pathway in a Rat Model of Cardiac Arrest and Cardiopulmonary Resuscitation.

Jingying Hou,Longyuan Jiang,Guanghui Zheng,Chaotao Zeng,Zhengfei Yang,Lian Liang

doi:10.3389/fphys.2021.689531

Abstract

In this study, we aimed to explore the role of lncRNAs in post-resuscitation myocardial dysfunction in a rat model of CA-CPR. A rat model of CA-CPR was constructed using a VF method. Myocardial functions, including cardiac output (CO), ejection fraction (EF), and myocardial performance index (MPI), were evaluated at the baseline, and 1, 2, 3, 4, and 6 h after resuscitation. A high throughput sequencing method was used to screen the differentially expressed lncRNAs, miRNAs, and mRNAs, which were further analyzed with bioinformatics. In addition, relationships between the molecules involved in the PI3K/Akt signaling pathway were explored with ceRNA network. Compared with the sham group, EF was significantly reduced and MPI was increased at the five consecutive time points in the CA-CPR group. 68 lncRNAs were upregulated and 40 lncRNAs were downregulated in the CA-CPR group, while 30 miRNAs were downregulated and 19 miRNAs were upregulated. Moreover, mRNAs were also differentially expressed, with 676 upregulated and 588 downregulated. GO analysis suggested that genes associated with cell proliferation, cell death and programmed cell death were significantly enriched. KEGG analysis showed that the PI3K/Akt, MAPK and Ras signaling pathways were the three most-enriched pathways. Construction of a ceRNA regulatory network indicated that LOC102549506, LOC103689920, and LOC103690137 might play important roles in the regulation of the PI3K/Akt signaling pathway in the CA-CPR treated rat. Taken together, LncRNAs, including LOC102549506, LOC103689920 and LOC103690137, might participate in post-resuscitation myocardial dysfunction by functioning as ceRNAs and regulating the PI3K/Akt signaling pathway.

Highlights

Cardiopulmonary resuscitation (CPR) after cardiac arrest (CA) can result in a variety of injuries such as severe ischemia, hypoxia and energy metabolism disorders (Sekhon et al, 2017)
We evaluated the relevant physiological and biochemical indexes in the two experimental groups, including cardiac output (CO), ejection fraction (EF), myocardial performance index (MPI), weight, Heart rates (HR), mean arterial pressures (MAP), PH, PaO2, lactate, ETCO2 and core temperature (Figure 1 and Table 1)
Compared to the sham group, EF was significantly reduced at the five consecutive time points in the CA-CPR group, whereas MPI was obviously increased correspondingly (P < 0.05, P < 0.05; Figure 1)

Summary

Introduction

Cardiopulmonary resuscitation (CPR) after cardiac arrest (CA) can result in a variety of injuries such as severe ischemia, hypoxia and energy metabolism disorders (Sekhon et al, 2017). Restoration of spontaneous circulation (ROSC) post CPR provokes serious reperfusion injury and further impairs myocardial function, which becomes the leading cause of death after resuscitation (Ha et al, 2017). High readmission and mortality rates were reported in the survived patients 3 years after hospital discharge. Cardiovascular and neurological factors were the primary cause of morbidity and mortality (Shuvy et al, 2017).

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