Abstract
BackgroundIt has been reported that the development of cervical squamous cell carcinoma (CSCC) requires the involvement of a large number of lncRNAs. In a recent study lncRNA, WT1-AS was been characterized as a tumor-suppressive lncRNA in gastric cancer. In our study we aim to explore the involvement of WT1-AS in CSCC.MethodsSeventy-six CSCC patients (20 to 63 years, 40.1 ± 6.1 year) from the 233 CSCC patients who were admitted by the Affiliated Tumour Hospital of Xinjiang Medical University between august 2010 and January 2014. RT-qPCR, cell proliferation rate measurement, cell transfection, and western blot were carried out to analyze the samples.ResultsWe found that HPV infection failed to affect WT1-AS expression in CSCC tissues, while WT1-AS was down-regulated in CSCC tissues compared with non-cancer tissues. P53 was also down-regulated in CSCC tissues and positively correlated with WT1-AS. Analysis of the survival of CSCC patients revealed that low levels of WT1-AS were accompanied by poor survival. Significantly up-regulated p53 was observed after WT1-AS over-expression in CSCC cells, while p53 over-expression failed to affect WT1-AS. P53 and WT1-AS over-expression resulted in the inhibited proliferation of CSCC cells.ConclusionTherefore, WT1-AS is down-regulated in CSCC and it may inhibit CSCC cell proliferation at least partially by up-regulating p53.
Highlights
It has been reported that the development of cervical squamous cell carcinoma (CSCC) requires the involvement of a large number of long non-coding RNAs (lncRNAs)
WT1-AS was affected by CSCC but not by human papillomavirus (HPV) infections WT1-AS in CSCC and non-cancer tissues of CSCC patients (n = 76) was detected by performing RT-qPCR
It was observed that expression levels of WT1-AS in CSCC tissues (Fig. 1a) and noncancer tissues (Fig. 1b) were not significantly different among 3 groups of patients
Summary
It has been reported that the development of cervical squamous cell carcinoma (CSCC) requires the involvement of a large number of lncRNAs. In a recent study lncRNA, WT1-AS was been characterized as a tumorsuppressive lncRNA in gastric cancer. Cervical cancer is a type of human cancer characterized by its high incidence and mortality rates [1]. It has been reported that cervical cancer cause about 300, 000 deaths every year worldwide [4]. For women aged between 20 and 39 years, cervical cancer is the second leading cause of cancer-related mortalities [5]. The high mortality rate and poor treatment outcomes are mainly caused by the unknown molecular mechanism of the pathogenesis. In-depth investigations on the molecular pathways involved in this disease are needed
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