Abstract
BackgroundLncRNA WT1-AS is a recently identified potential tumor suppressor in gastric cancer. This study mainly explored the role of WT1-AS in non-small cell lung cancer (NSCLC).MethodsWT1-AS and TGF-β1 mRNA in two types of tissues of 74 NSCLC patients were detected by performing RT-qPCR experiments. WT1-AS and TGF-β1 expression vectors were established using the pcDNA3.1 vector. Protein concentration was measured by BCA assay. Mean values in this study were calculated using the data of three biological replicates of each experiment.ResultsWe found that WT1-AS was down-regulated, while TGF-β1 was upregulated in NSCLC tissues. Survival analysis showed that low levels of WT1-AS expression predicted poor survival of NSCLC patients. WT1-AS and TGF-β1 were inversely correlated in NSCLC tissues. Over-expression experiments revealed down-regulated TGF-β1 after WT1-AS over-expression, while TGF-β1 over-expression failed to affect WT1-AS. WT1-AS over-expression resulted in inhibited cancer cell stemness. TGF-β1 over-expression played an opposite role and attenuated the effects of TGF-β1 over-expression.ConclusionTherefore, WT1-AS over-expression may inhibit non-small cell lung cancer cell stemness by down-regulating TGF-β1.Trial registrationThe First Affiliated Hospital of Anhui Medical University Ethics committee approved this study (AHMU20101009).
Highlights
LncRNA WT1-AS is a recently identified potential tumor suppressor in gastric cancer
WT1-AS and TGF-β1 mRNA showed opposite expression pattern in non-small cell lung cancer (NSCLC) WT1-AS and TGF-β1 mRNA in two types of tissues (NSCLC and non-cancer tissues) of 74 NSCLC patients were detected by performing RT-qPCR experiments
It was found that the expression levels of WT1-AS were significantly lower (Fig. 1a, p < 0.05), while expression levels of TGF-β1 were substantially higher (Fig. 1b, p < 0.05) in NSCLC tissues compared to non-cancer tissues
Summary
LncRNA WT1-AS is a recently identified potential tumor suppressor in gastric cancer. This study mainly explored the role of WT1-AS in non-small cell lung cancer (NSCLC). Non-small-cell lung cancer (NSCLC) is the primary subtype of lung cancer and account for more than 85% of all lung cancer cases. Altered expression of genetic factors, such as tumor suppressors and oncogenes plays pivotal roles in NSCLC [7, 8]. RNAs (ncRNAs), such as its subgroup long ncRNAs (> 200 nt, lncRNAs) participate in human cancer, including NSCLC by regulating the expression of the protein-coding gene [9]. A particular lncRNA, which is named WT1-AS, attracted our attention This lncRNA plays tumor-suppressive roles in many types of cancer, such as gastric cancer [10], cervical cancer [11] and hepatocellular carcinoma [12], while its role in NSCLC is unknown. The present study was carried out to test this hypothesis
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