Abstract
The function and possible mechanism of lncRNA Small Nucleolar RNA Host Gene 3 (SNHG3) in GC have not been fully studied. The aim of our study was to investigate the role of SNHG3 in the proliferation, migration, and invasion of GC cell lines. The expressions of SNHG3, miR-326, and TWIST in GC9811-P GC cell lines were detected by RT-qPCR. Western blotting was performed to detect the protein levels of TWIST and EMT-related genes. Luciferase reporter gene analysis and RNA immunoprecipitation (RIP) analysis confirmed the interaction between lncRNA SNHG3, miR-326, and TWIST. CCK-8 and Transwell assays were performed to detect cell proliferation, invasion, and migration abilities. The results showed that lncRNA SNHG3 and TWIST were highly expressed in GC cell lines, while miR-326 was expressed to a low degree. Moreover, lncRNA SNHG3 knockdown or miR-326 overexpression significantly inhibited cell proliferation, migration, and invasion of GC cell lines. In addition, TWIST overexpression can reverse the inhibition of lncRNA SNHG3 knockdown or miR-326 overexpression on cell proliferation, migration, and invasion. In conclusion, lncRNA SNHG3 may promote GC progression through the miR-326/TWIST axis, which may provide a new diagnostic and prognostic biomarker for GC.
Highlights
Gastric cancer (GC) is a common malignancy in the world [1]. e most common cause of GC is Helicobacter pylori infection, accounting for more than half of the incidence
Small Nucleolar RNA Host Gene 3 (SNHG3), miR-326 and TWIST were detected in GES-1, HGC-27, and GC9811-P cell lines. e results showed that the expression of Long noncoding RNA (lncRNA) SNHG3 and TWIST in HGC-27 and GC9811-P cell lines was higher than control group (Figures 1(a)–1(c))
The expression of miR326 in HGC-27 and GC9811-P cell lines was obviously lower than that in GES-1 cell line (Figure 1(d)). ese results indicated that the unusual expression of lncRNA SNHG3, miR-326, and TWISTmight be related to the progress of GC
Summary
Gastric cancer (GC) is a common malignancy in the world [1]. e most common cause of GC is Helicobacter pylori infection, accounting for more than half of the incidence. Gastric cancer (GC) is a common malignancy in the world [1]. Other recognized risk factors include smoking, the intake of pickled vegetables in the diet, and the clinical management of obesity [2]. E treatment of GC mainly includes surgery, chemotherapy, radiotherapy, and targeted therapy [3]. SNHG3 is a new type of lncRNA, which may be related to Alzheimer’s disease and colorectal cancer. E results showed that the expression of Small Nucleolar RNA Host Gene 3 (SNHG3) was related to the malignant state, and the prognosis was relatively poor. SNHG3 was identified as a competitive endogenous RNA molecule to promote the malignant progression of colorectal cancer [8]. E abnormal upregulation of snhg in ovarian cancer is closely related to poor prognosis and malignant progression [9]. LncRNA SNHG3 is characterized by its involvement in the microRNA pathway of HCC, where miR-128/CD151 signaling of SNHG3 in-
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