Abstract
Background: Glioma is the most common malignant tumor in the human central nervous system. Long noncoding RNA nuclear paraspeckle assembly transcript 1 (NEAT1) promotes oncogenesis in various tumors. In the present study, we aimed to examine the role of NEAT1 in altering the properties of gliomas.Methods: Quantitative real-time PCR technology was used to determine the expression levels of relevant genes in tumor tissues and cell lines. The protein expression levels were validated by Western blotting. Cell counting kit-8 (CCK-8) and colony formation assays were used to test the cell proliferation ability. A luciferase reporter assay was used to determine the interactions of the genes. Tumor xenografts were used to detect the role of NEAT1 in gliomas in vivo.Results: We demonstrated that NEAT1 up-regulated glioma cells and negatively correlated with miR-98-5p in glioma tissues. A potential binding region between NEAT1 and miR-98-5p was confirmed by dual-luciferase assays. NEAT1 knockdown inhibited glioma cell proliferation. The inhibition of miR-98-5p rescued the knockdown of NEAT1 in glioma cells. Basic leucine zipper and W2 domain containing protein 1 (BZW1) was identified as a direct target of miR-98-5p. We also identified that BZW1 was positively correlated with NEAT1 in glioma tissues. NEAT1 knockdown inhibited glioma cell proliferation in vivo via miR-98-5p/BZW1.Conclusion: Our results suggest that NEAT1 plays an oncogenic function in glioma progression. Targeting NEAT1/miR-98-5p/BZW1 may be a novel therapeutic treatment approach for glioma patients.
Highlights
Glioma is the most common malignant tumor that can be found in the central nervous system of humans [1,2]
nuclear-enriched abundant transcript 1 (NEAT1) is negatively correlated with miR-98-5p in glioma tissues
LncRNA NEAT1 was up-regulated in glioma tissues compared with that in peritumor tissues (Figure 1A)
Summary
Glioma is the most common malignant tumor that can be found in the central nervous system of humans [1,2]. Aberrant overexpression of the long noncoding RNA nuclear-enriched abundant transcript 1 (NEAT1) has been reported in many types of solid tumors, such as lung cancer, esophageal cancer, colorectal cancer, hepatocellular carcinoma and glioma. There are few studies about the role of nuclear-enriched abundant transcript 1 (NEAT1) in glioma. Long noncoding RNA nuclear paraspeckle assembly transcript 1 (NEAT1) promotes oncogenesis in various tumors. Methods: Quantitative real-time PCR technology was used to determine the expression levels of relevant genes in tumor tissues and cell lines. Results: We demonstrated that NEAT1 up-regulated glioma cells and negatively correlated with miR-98-5p in glioma tissues. The inhibition of miR-98-5p rescued the knockdown of NEAT1 in glioma cells. NEAT1 knockdown inhibited glioma cell proliferation in vivo via miR-98-5p/BZW1. Targeting NEAT1/miR-98-5p/BZW1 may be a novel therapeutic treatment approach for glioma patients
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