Abstract
The aim of this study was to investigate the roles of MT1JP and β-catenin in retinoblastoma. We performed quantitative Reverse Transcriptase-Polymerase Chain Reaction (qRT-PCR) to quantify the expressions of MT1JP and β-catenin in 44 retinoblastoma tissues and matched non-tumor tissues. What's more, retinoblastoma cell lines were transfected with pcDNA3.1-MT1JP, after which proliferation, cell cycle, apoptosis, and expression of β-catenin as well as its downstream targets were assayed. We also conducted TOP-Flash reporter assay to explore the activity of Wnt/β-catenin signaling pathway. The results revealed that MT1JP was down-regulated, while β-catenin was highly expressed in retinoblastoma cells. Meanwhile, the forced expression of MT1JP impaired the expression of the β-catenin protein and its downstream targets such as cyclin D1, c-myc. We demonstrated that MT1JP was a tumor suppressor by negatively modulating the activity of the Wnt/β-catenin signaling pathway in the development of retinoblastoma and might function as a prognostic biomarker and therapeutic target.
Published Version
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