Abstract
BackgroundCurrently, several non-coding RNAs (ncRNAs) were distinguished in polycystic ovarian syndrome (PCOS). This present study aims to explore the potential function of lncRNA HOTAIRM1/miR-433-5p/PIK3CD in ovarian granulosa cells.MethodsWe analyzed the expression profiles of HOTAIRM1, miR-433-5p and PIK3CD in PCOS samples by enquiring GEO database. GSEA was applied to enrich the pathways related to PCOS. The target association between HOTAIRM1 and miR-433-5p or the binding association between miR-433-5p and PIK3CD were assessed by online prediction tools and a dual luciferase reporter assay. qPCR and western blotting assays were used to detect PIK3CD expression after HOTAIRM1 and miR-433-5p treatment. The proliferation and apoptosis of ovarian granulosa cells were estimated by cell counting kit-8 and flow cytometry assays, respectively.ResultsThe expression profiles of HOTAIRM1 and PIK3CD were increased, whereas miR-433-5p was decreased in PCOS tissues. PIK3CD expression was positively regulated by HOTAIRM1 and negatively modulated by miR-433-5p. Overexpression of HOTAIRM1 reduced the proliferative ability and increased the apoptotic ability of granulosa cells, whereas upregulation of miR-433-5p or downregulation of PIK3CD reversed the effects of HOTAIRM1 on granulosa cells. Moreover, overexpression of miR-433-5 displayed a results with increasing proliferative ability and decreasing apoptotic ability, but upregulation of PIK3CD eliminated the function of miR-433-5p on granulosa cells.ConclusionsOur findings illustrated that HOTAIRM1 could sponge miR-433-5p to promote PIK3CD expression, thereby regulating the growth and apoptosis of granulose cells in PCOS.
Highlights
Polycystic ovarian syndrome (PCOS), as the most common endocrine disorder in women during their reproductive ages, is associated with chronic anovulation and androgen overproduction [2]
High expression of HOTAIRM1 suppressed the viability of KGN cells By consulting GSE34526, we observed that HOTAIRM1 was highly expressed in polycystic ovarian syndrome (PCOS) patients (n = 7) compared with the normal samples (n = 3, Fig. 1a)
Afterwards, we found that the viability of KGN cells was decreased significantly when HOTAIRM1 was upregulated (Fig. 1d), indicating that HOTAIRM1 affected the function of KGN cells
Summary
Polycystic ovarian syndrome (PCOS), as the most common endocrine disorder in women during their reproductive ages, is associated with chronic anovulation and androgen overproduction [2]. PCOS is involved in diverse metabolic perturbations, including. Granulosa cells are somatic cells of the sex cord, which are related with the progression of oocytes in mammalian ovaries [1]. Granulosa cells are involved in numerous ovary-related diseases, including PCOS [30]. Recent findings have suggested that several non-coding RNAs regulated these female reproductive system dysfunctions [23]. Several non-coding RNAs (ncRNAs) were distinguished in polycystic ovarian syndrome (PCOS). This present study aims to explore the potential function of lncRNA HOTAIRM1/miR-433-5p/PIK3CD in ovarian granulosa cells
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