The LINC00477/miR-128 axis promotes the progression of polycystic ovary syndrome by regulating ovarian granulosa cell proliferation and apoptosis

Haijie Gao,Jiying Chen,Jinna Jiang,Yingying Shi,Lijian Zhao,Chenhong Wang

doi:10.1186/s12958-021-00718-z

Abstract

BackgroundLong noncoding RNAs (lncRNAs) participate in the pathogenesis of various human diseases. This study aims to investigate the roles of lncRNA LINC00477 in polycystic ovary syndrome (PCOS), especially the impacts of LINC00477 on the proliferation and migration of human granulosa cells and the related mechanisms.MethodsqRT-PCR analysis was performed to examine the expression pattern of LINC00477 in serum samples of PCOS patients as well as PCOS animal models. The effect of LINC00477 on the viability and apoptosis of ovarian granulosa cells was detected by MTT and flow cytometry assays. The correlation between LINC00477 and miR-128 was verified by bioinformatics analysis and dual-luciferase reporter and RNA pull-down assays. Finally, rescue assays were performed to analyze the effects of the LINC00477-miR-128 axis on the biological behaviors of granulosa cells.ResultsLINC00477 was significantly upregulated in the serum of PCOS patients as well as PCOS mouse models. LINC00477 overexpression inhibited the proliferation and promoted the apoptosis of granulosa cells, whereas knockdown of LINC00477 yielded the opposite effects. Moreover, miR-128 mimics partially abrogated the effect of LINC00477 on granulosa cells.ConclusionLINC00477 may function as a ceRNA to inhibit proliferation and apoptosis of granulosa cells by modulating miR-128 expression.

Highlights

Polycystic ovary syndrome (PCOS) is a commonly diagnosed reproductive disease with a high incidence rate, and polycystic ovary syndrome (PCOS) is one of the leading causes of infertility worldwide [1,2,3]
We found that LINC00477 expression was markedly increased in the serum of PCOS patients compared with healthy controls
Increased LINC00477 expression in PCOS mouse models the roles of LINC00477 in PCOS were explored by mouse PCOS models in vivo

Summary

Introduction

Polycystic ovary syndrome (PCOS) is a commonly diagnosed reproductive disease with a high incidence rate, and PCOS is one of the leading causes of infertility worldwide [1,2,3]. Research on the roles of noncoding RNAs in different diseases has become a hot field. With in-depth investigations of the underlying mechanism of lncRNAs, researchers found that lncRNAs can regulate various biological events, such as cell proliferation, migration, and differentiation, by affecting gene expression at the posttranscriptional level. Long noncoding RNAs (lncRNAs) participate in the pathogenesis of various human diseases. This study aims to investigate the roles of lncRNA LINC00477 in polycystic ovary syndrome (PCOS), especially the impacts of LINC00477 on the proliferation and migration of human granulosa cells and the related mechanisms

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Conclusion