Abstract
BackgroundCervical cancer (CC) is one of the most common and malignant tumors in women. In this study, we aim to explore the role and mechanism of F-box and leucine rich repeat protein 19 antisense RNA 1 (FBXL19-AS1), a novel long-chain non coding RNA (lncRNA) with marked roles in a variety of tumors, in regulating the proliferation and metastasis of CC.MethodsThe expression of FBXL19-AS1, miR-193a-5p and COL1A1 were detected by RT-PCR and western blot. Gain- and loss-of functional assays of FBXL19-AS1 and miR-193a-5p were performed in CC cell lines in vitro or in vivo. The proliferation, migration, invasion, apoptosis and epithelial-mesenchymal transition (EMT) of CC cells were determined.ResultsFBXL19-AS1 and COL1A1 were significantly up-regulated in CC tissues, while miR-193a-5p was significantly down-regulated. Overexpression of FBXL19-AS1 significantly promoted the proliferation, migration, invasion, EMT and growth of CC cells and inhibited apoptosis, while knockdown of FBXL19-AS1 had the opposite effects. On the other hand, miR-193a-5p inhibited the proliferation and metastasis of CC cells. Mechanistically, FBXL19-AS1 functioned as a competitive endogenous RNA (ceRNA) and inhibited the expression of miR-193a-5p, which targeted at the 3’-UTR site of COL1A1 and negatively regulated COL1A1 expression.ConclusionsFBXL19-AS1 promotes the proliferation and metastasis of CC cells by sponging miR-193a-5p and up-regulating COL1A1.
Highlights
Cervical cancer (CC) is one of the most common and malignant tumors in women
FBXL19‐AS1 was upregulated in CC tissues and modulated CC cell proliferation and apoptosis
The results indicated that FBXL19AS1 was notably increased in CC tissues compared with that of adjacent normal tissues (p < 0.05, Fig. 1A) and CC cells compared with HUCEC cells (p < 0.05, Fig. 1B)
Summary
We aim to explore the role and mechanism of F-box and leucine rich repeat protein 19 antisense RNA 1 (FBXL19-AS1), a novel long-chain non coding RNA (lncRNA) with marked roles in a variety of tumors, in regulating the proliferation and metastasis of CC. Long non-coding RNAs (lncRNAs) are non-coding RNAs with more than 200 nt in length and function as gene expression regulators that play a vital role in regulating cell metabolism and growth [3]. LncRNAs affects the apoptosis, invasion and metastasis of tumor cells and have a significant influence on tumor development [4]. FBXL19-AS1 expression is remarkably increased in breast cancer and promotes its development via promoting proliferation and reducing cell apoptosis [7]. The functions and mechanisms of lncRNA FBXL19-AS1 in cervical cancer are still elusive
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have