Effects of long-chain non-coding RNA MCM3AP-AS1 targeting RNA-876-5p on proliferation and metastasis of ovarian cancer cells

Abstract

Objective To investigate the effects of long-chain non-coding RNA MCM3AP-AS1 (MCM3AP antisense RNA 1, MCM3AP-AS1) targeting regulation of microRNA-876-5p (microRNA-876-5p) on the proliferation and metastasis of ovarian cancer cells. Methods Forty patients with ovarian cancer were selected from Apr. 2017 to May. 2018. The ovarian cancer cell lines (CaOV3, SKOV3, OVCAR3, OV90) and normal ovarian epithelial cell lines (HOSE) were also selected. Real-time quantitative polymerase chain reaction (qRT-PCR) was used to detect the expression of lncRNA MCM3AP-AS1 in ovarian cancer tissues and cells. A low expression model of lncRNA MCM3AP-AS1 was established. Proliferation and metastasis of ovarian cancer cells were detected by MTT and transwell. Luciferase assay and qRT-PCR were used to verify the targeting relationship between lncRNA MCM3AP-AS1 and miR-876-5p. Results The level of lnRNA MCM3AP-AS1 in ovarian cancer tissue was (8.45±0.86) , higher than that in adjacent tissue (4.45±0.45) ; The levels in ovarian cancer cells were CaOV3 (1.53±0.12) , SKOV3 (1.82±0.23) , OVCAR3 (1.34±0.12) , higher than those in normal ovarian epithelial cell HOSE (1.00±0.10) ; Down-regulated lncRNA MCM3AP-AS1 could reduce the proliferation and metastasis of ovarian cancer cells; lncRNA MCM3AP-AS1 could interact with and down-regulate the expression of miR-876-5p. Conclusion LncRNA MCM3AP-AS1 can promote the proliferation, metastasis and invasion of ovarian cancer cells by targeting the inhibition of microRNA-876-5p. Key words: LncRNA MCM3AP-AS1; MiR-876-5p; Ovarian cancer BxPC-3 cells; Proliferation; Metastasis