Abstract
BackgroundLncRNA DLGAP1-AS2 plays an oncogenic role in glioma, while its role in other cancers is unknown. This study aimed to study the role of DLGAP1-AS2 in non-small cell lung cancer (NSCLC).MethodsExpression of DLGAP1-AS2 in NSCLC and paired non-tumor tissues from 64 NSCLC patients and the prognostic value of DLGAP1-AS2 for NSCLC were analyzed by performing a 5-year follow-up study. The interaction between DLGAP1-AS2 and miR-503 was confirmed by dual luciferase reporter assay, and their relationship was explored in NSCLC cells transfected with DLGAP1-AS2 expression vector or miR-503 mimic. The roles of DLGAP1-AS2 and miR-503 in regulating cyclin D1 expression were analyzed by RT-qPCR and Western blot. Cell proliferation was analyzed by CCK-8 assay.ResultsDLGAP1-AS2 was upregulated in NSCLC and predicted poor survival. Interaction between DLGAP1-AS2 and miR-503 was confirmed by dual luciferase activity assay. Overexpression experiments showed that DLGAP1-AS2 and miR-503 overexpression failed to significantly affect the expression of each other. Interestingly, DLGAP1-AS2 overexpression upregulated cyclin D1, a target of miR-503, increased cell proliferation and reduced the effects of miR-503 overexpression on cyclin D1 expression and cell proliferation.ConclusionsDLGAP1-AS2 may regulate miR-503/cyclin D1 to promote cell proliferation in NSCLC.
Highlights
LncRNA DLGAP1-AS2 plays an oncogenic role in glioma, while its role in other cancers is unknown
This study aimed to explore the interactions among DLGAP1-AS2, miR-503, and cyclin D1 in non-small cell lung cancer (NSCLC)
DLGAP1‐AS2 was overexpressed in NSCLC and correlated with the poor survival of NSCLC patients DLGAP1-AS2 expression in NSCLC tissues and their paired non-tumor tissues collected from 64 patients was determined by reverse transcriptions (RTs)-qPCR
Summary
LncRNA DLGAP1-AS2 plays an oncogenic role in glioma, while its role in other cancers is unknown. Lung cancer is the major cause of cancer deaths among both females and males worldwide [1, 2]. It accounted for about 11.6% of all new cancer cases and caused 18.4% of all cancer deaths in 2018 [3]. Tumor metastasis to distant organs, such as the brain, bones, and liver, is common in lung cancer patients [5]. With our understanding of the molecular mechanisms of lung cancer increasing, novel therapies, such as targeted therapy that can be applied to suppress tumor growth and metastasis, have been developed [9, 10].
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
