LncRNA CASC15 upregulates cyclin D1 by downregulating miR-365 in laryngeal squamous cell carcinoma to promote cell proliferation

Minhui Zhu,Hongliang Zheng,Shicai Chen,Caiyun Zhang,Peng Zhou

doi:10.1186/s40463-022-00560-2

Abstract

BackgroundThis study investigated the role of lncRNA CASC15 in laryngeal squamous cell carcinoma (LSCC).MethodsThis study included 58 LSCC patients. Both tumor (LSCC) and adjacent (within 3 cm around tumors) non-tumor tissues from 3 different sites of each patient were collected. CCK-8 assay was used to determine cell proliferation. The expression levels of proteins and mRNAs were determined by Western blotting analysis and qRT-PCRs, respectively.ResultsCASC15 was upregulated in LSCC and high expression levels of CASC15 predicted poor survival. In LSCC tissues, CASC15 was negatively correlated with miR-365 but positively correlated with cyclin D1. In LSCC cells, overexpression of CASC15 resulted in downregulation of miR-365 and upregulation of cyclin D1. Overexpression of miR-365 did not affect the expression of CASC15 but downregulated cyclin D1. Overexpression of Cyclin D1 did not affect the expression of miR-365 and CASC15. Overexpression of CASC15 and cyclin D1 led to promoted, while overexpression of miR-365 led to inhibited LSCC cell proliferation. In addition, overexpression of miR-365 reduced the effects of overexpression of CASC15.ConclusionTherefore, CASC15 upregulates cyclin D1 by downregulating miR-365 in LSCC to promote cell proliferation.

Highlights

Laryngeal cancer is the most common type of head and neck cancers
Our preliminary deep sequencing data showed that CASC15 was upregulated in laryngeal squamous cell carcinoma (LSCC) and positively correlated with cyclin D1, which mediates cell cycle progression and has critical roles in tumor growth [12]
CASC15 was correlated with miR‐365 and cyclin D1 in LSCC The expression of miR-365 and Cyclin D1 in both types of tissue were detected by RT-qPCR

Summary

Introduction

Laryngeal cancer is the most common type of head and neck cancers. The most common pathological type of laryngeal cancer is laryngeal squamous cell carcinoma (LSCC), which accounts for about 85–96% of laryngeal cancer [1]. Our preliminary deep sequencing data showed that CASC15 was upregulated in LSCC and positively correlated with cyclin D1 (data not shown), which mediates cell cycle progression and has critical roles in tumor growth [12]. Our deep sequencing data showed the inverse correlation between CASC15 and miR-365 (data not shown). It has been reported that miR-365 can directly target cyclin D1, which plays critical roles in almost all types of cancer including LSCC [13]. This study investigated the role of lncRNA CASC15 in laryngeal squamous cell carcinoma (LSCC)

Methods

Results

Conclusion