Abstract
Cyclopropenoid fatty acids in the diet of rainbow trout caused significant reductions in liver protein and activity of glucose-6-phosphate dehydrogenase, NADP-linked isocitrate dehydrogenase, lactate dehydrogenase, and malate dehydrogenase. Changes in total activity were usually accompanied by similar changes in specific activity. The activity of glucose-6-phosphate dehydrogenase appeared to be more sensitive to the ingestion of cyclopropenoid fatty acids than the other dehydrogenases studied. Feeding 20 ppb aflatoxin B(1) to rainbow trout did not significantly change the activity of the dehydrogenases except for a small increase in the activity of glucose-6-phosphate dehydrogenase after 21 days of feeding. Relationships of these changes to the cocarcinogenicity of cyclopropenoid fatty acids and the carcinogenicity of aflatoxin are discussed.
Highlights
Cyclopropenoid fatty acids in the diet of rainbow trout caused significant reductions in liver protein and activity of glucose-6-phosphate dehydrogenase, NADP-linked isocitrate dehydrogenase, lactate dehydrogenase, and malate dehydrogenase
Presence of glycogen deposits in livers of Cyclopropenoid fatty acids (CPFA)-fed trout suggested that these cyclic fatty acids interfered with normal carbohydrate metabolism
To aid in understanding this phenomenon, the present study was undertaken to determine the effect of CPFA on several liver dehydrogenases involved in carbohydrate metabolism
Summary
Rainbow trout (Sulmo gairdneri) of the Mt. Shasta strain were spawned, reared, and maintained as described by Lee et al [5]. Fingerlings were fed a semipurified test diet [5] for at least 90 days prior to initiation of the experiment. 8 times a day throughout the growth period and experimental trials. The homogenate was centrifuged at 2500 g for 10 min at 4°C. The resulting supernate was used as enzyme source for the following studies. This procedure was used throughout the study
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