Abstract
Water-in-water (W/W) emulsion is an interesting template for microentrapment of hydrophilic compounds due to biocompatible nature of its aqueous media. W/W emulsification can be established from a destabilised aqueous two-phase system (ATPS). In this study, binodal data of ATPSs comprising polyethylene glycol (PEG) and gelatin were generated. Binodal curves of PEG + gelatin + water systems were successfully correlated using non-linear empirical Merchuk equation, and the tie lines were constructed using lever-arm rule. The effects of temperature, type of gelatin, and molecular weight of PEG on the binodal curves were evaluated. Notably, a higher molecular weight of PEG decreased the concentration of gelatin required for two-phase formation. ATPSs made of gelatin bovine has a larger biphasic region than that of gelatin porcine. Gelatin microgel was successfully synthesized from the PEG + gelatin emulsion system via rapid cooling of the dispersed phase of gelatin. The size of gelatin microgel was reduced by a higher stirring speed, while a temperature as high as 333.15 K increased the size distribution of gelatin microgel. Lastly, the microentrapment of model protein, green fluorescent protein, was demonstrated. PEG/Gelatin system holds good potential for food engineering and delivery system.
Published Version
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