Abstract
The retention behaviour of several β-lactam antibiotics in reversed-phase liquid chromatography with mobile phases containing crown ether(18-crown-6 or dicyclohexyl-18-crown-6) has been investigated. The capacity factors were determined with various concentrations of crown ether and methanol in the aqueous mobile phase at different pH values. The observed profiles of capacity factor, k, vs. crown ether concentration and k vs. pH are discussed with reference to an equation derived from a chromatographic model involving association of β-lactam antibiotics with crown ethers and bindings of free and associated species to the hydrophobic stationary phase. The effect of ionic salts on the capacity factor is also discussed. The applicability of the method is demonstrated by specific separations of ampicillin in urine and cephalexin in plasma.
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