Abstract
The amounts of water which exhibit selectivity to solutes in water-swollen hydrophilic polymer gel packings were determined by a liquid chromatographic method designed on the basis of the mobile phase electrolyte effects on the retention of ionic solutes. The estimated amounts of the water in three types of water-swollen hydrophilic polymer gels, a cross-linked dextran, poly(vinyl alcohol) and polyacrylamide, agree well with the sum of the amount of freezable bound water and that of non-freezing water determined by means of differential scanning calorimetry. Retention selectivities of these packings were evaluated based on the plots of logarithmic distribution coefficients, ln K D, of various organic compounds obtained on one packing versus those on another with the same mobile phase, water. It was found that the ln K D vs. ln K D plots between two packings of the same polymer matrix with different degrees of cross-linking were linear and the slopes of the plots depended on the fractions of the freezable bound water and the non-freezing water in the stationary phase; the water-swollen hydrophilic polymer packings which contained a smaller fraction of the freezable bound water exhibited larger retention selectivities.
Published Version
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