Abstract
An HPLC procedure was developed for the simultaneous determination of salicylazosulphapyridine, and its main metabolites 5-aminosalicylic acid and sulphapyridine, in human serum and synovial fluid. The analytical procedure consisted of a single ion-pair extraction step for an Extrelut column with methylene chloride. The investigated compounds and the added sulphadimidine internal standard were eluated from a Hypersil-MOS reversed-phase column by stepwise gradient; mobile phase was methanol-0.01 M potassium dihydrogenphosphate (3:7, 0.0–2.0 min and 8:2, 2.1–6.5 min).
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