Abstract
An HPLC assay was developed for the determination of sulphasalazine (salicylazosulphapyridine), and its main metabolites; sulphapyridine, acetylsulphapyridine, hydroxysulphapyridine and acetylhydroxysulphapyridine in human serum, synovial fluid and urine. Sep-Pak C18 cartridge was used for the extraction of the investigated compounds and the added sulphadimidine internal standard. The methylene chloride-chloroform-methanol eluate was evaporated and the dissolved dry residue was injected onto a Hypersil ODS (100×2.1 mm) 5 μm “microbore”, analytical column. A linear gradient was used, the mobile phase was ternary. The assay is sufficiently sensitive for routine therapeutic drug monitoring and phenotyping in patients treated with sulphasalazine.
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