Abstract
A simple and rapid high performance liquid chromatographic (HPLC) determination of residual penicillin G (benzylpenicillin, PCG) in cattle, pig, and chicken tissues (muscle, kidney, and liver) was developed. The sample preparation was performed by homogenizing with 0.1 M phosphate buffer (pH 6.5) followed by an Ultrafree®-MC/PL as a centrifugal ultrafiltration unit. For determination/identification of PCG, a Mightysil® RP-4GP column and a mobile phase of ethanol—0.1 M phosphate buffer (pH 6.5) (1:4, v/v) with a photodiode array detector was used. The average recoveries from spiked PCG (0.05, 0.1, 0.5, and 1.0 ppm) were in excess of 78.8% with coefficients of variation between 1.6 and 4.9%. The limit of detection was 0.04 ppm. The total time and solvent required for the analysis of one sample was <40 min and <2 mL of ethanol, respectively.
Published Version
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