Abstract
Abstract A simple and rapid liquid chromatographic (LC) method was used for simultaneous determination of ammonia, cadaverine, histamine, methylamine, putrescine, spermidine, spermine, tryptamine, and tyramine. These amines were separated as dansyl chloride derivatives in 22 min on a reversed-phase LC column using a binary elution gradient (water and acetonitrile). Three species of fish (plaice, whiting, and herring) stored in ice at 0C were analyzed to determine new decomposition indexes (ratios), which are less species-dependent than measurements of total volatile basic nitrogen (TVBN), trimethylamine (TMA), and P % (TMA/TVBN).
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