Abstract
Electrospinning has become a popular nanotechnology for the fabrication of tissue engineering scaffolds, which can precisely regulate fiber diameter and microstructure. Herein, we have prepared a three-dimensional polyacrylonitrile (PAN) nanofiber by liquid-assisted electrospinning. The spacing between PAN nanofibers can reach to 15-20 μm, as the uniform internally connected pore structure can be formed, through the regulation of parameters. Furthermore, the chitosan attached to the as-prepared nanofibers gives the material antibacterial effect and increases its biocompatibility. Meanwhile, the special structure of chitosan also provides the possibility for further loading drugs in dressings in the future. This newly developed nanocomposite seems to be highly suitable for wound healing due to its unique properties of biodegradability, biocompatibility, and antimicrobial effectiveness.
Highlights
As the largest organ of the human body, the skin is the first barrier for the human body to protect itself
The 3T3 fibroblast cells were cultured on coated nanofiber scaffolds (CNSs) with different concentrations of chitosan solution, as the cells cultured on tissue culture plates (TCPs) in the microplate were tested as the blank control
The three-dimensional fluffy nanofiber sponges (FNFSs) appeared as fibers with interconnected pores in the liquid environment (80% ethanol), presenting properties that were not available in two-dimensional fiber membranes, which provided good application prospect in adsorption materials, tissue engineering, and other fields
Summary
As the largest organ of the human body, the skin is the first barrier for the human body to protect itself. As a biomaterial with rich pore structure, electrospinning nanofibers can be used as scaffold for three-dimensional cell culture in tissue engineering [21]. Due to their porous structure which provides sufficient space for cells’ uniform distribution and facile delivery of oxygen and nutrients, the 3D nanofiber can mimic the topology and biological functions of the extracellular matrix (ECM) for guided cell growth. The 3D network structure was formed by the stacking of one-dimensional nanowires, and the amino group of chitosan was used to complete the crosslinking to enhance the antibacterial property of the material. The appropriate amount of electrospinning nanofibers was allocated to the 12-hole plate or 48-hole plate and freeze dried overnight to obtain the fluffy nanofiber sponges (FNFSs)
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