Abstract
1. 1. The net 42K +uptake by isolated mouse fat cells has been studied. 2. 2. 42K + was found to accumulate in fat cells by an energy-requiring process that was stimulated by Na + and inhibited by ouabain at concentrations as low as 1 μM. 3. 3. Stimulation of lipolysis by adrenaline, by ACTH or by xanthine derivatives resulted in a marked decrease in 42K +uptake by fat cells. The magnitude of this inhibition was proportional to that of lipolysis. 4. 4. Insulin, even in the absence of added glucose, markedly decreased the hormone- or xanthine derivative-induced lipolysis. Concomitantly, insulin prevented the decrease in 42K + uptake induced by these lipolytic agents. 5. 5. It is suggested that a decrease in energy availability and/or production occurs during lipolysis, and that it is partly responsible for the observed decrease in net 42K + uptake. Furthermore it is thought that insulin, in the absence of added glucose, prevents such a decrease in energy availability and/or production via its antilipolytic action, thereby counteracting the drop in 42K + uptake induced by lipolytic hormones and agents.
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