Lipoaspirate Shows In Vitro Potential for Wound Healing.

Chiara Ceresa,Carlo Tremolada,Alice Marchetti,Alessia Borrone,Maurizio Rinaldi,Letizia Fracchia,Michela Bosetti

doi:10.3390/pharmaceutics14020447

Chiara Ceresa, Carlo Tremolada + Show 5 more

Open Access

https://doi.org/10.3390/pharmaceutics14020447

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Journal: Pharmaceutics	Publication Date: Feb 19, 2022
Citations: 5	License type: CC BY 4.0

Affiliation: University of Eastern Piedmont Amadeo Avogadro

Abstract

Mesenchymal stem cells (MSCs) are a promising therapy in wound healing, although extensive time and manipulation are necessary for their use. In our previous study on cartilage regeneration, we demonstrated that lipoaspirate acts as a natural scaffold for MSCs and gives rise to their spontaneous outgrowth, together with a paracrine effect on resident cells that overcome the limitations connected to MSC use. In this study, we aimed to investigate in vitro whether the microfragmented adipose tissue (lipoaspirate), obtained with Lipogems® technology, could promote and accelerate wound healing. We showed the ability of resident cells to outgrow from the clusters of lipoaspirate encapsulated in a 3D collagen substrate as capability of repopulating a culture of human skin. Moreover, we demonstrated that the in vitro lipoaspirate paracrine effect on fibroblasts and keratinocytes proliferation, migration, and contraction rate is mediated by the release of trophic/reparative proteins. Finally, an analysis of the paracrine antibacterial effect of lipoaspirate proved its ability to secrete antibacterial factors and its ability to modulate their secretion in culture media based on a bacterial stimulus. The results suggest that lipoaspirate may be a promising approach in wound healing showing in vitro regenerative and antibacterial activities that could improve current therapeutic strategies.

Highlights

Skin, being the outermost organ that covers the entire surface of the human body, is very common to injury
In our previous study on cartilage repair [13], we demonstrated that these limitations can be overcome, since lipoaspirate acts as a natural scaffold rich in Mesenchymal stem cells (MSCs) and gives rise to spontaneous cell outgrowth
In addition to the increased antibacterial effect against E. coli and P. aeruginosa of both conditioned media obtained after bacterial stimulation, we demonstrated, for the first time, that the conditioned medium of stimulated lipoaspirate was significantly more effective than that of stimulated Human adipose-derived stem cells (hADSCs), both against

Summary

Introduction

Skin, being the outermost organ that covers the entire surface of the human body, is very common to injury. Traditional wound management options include regular dressings and skin grafting. Skin grafting, which is the transfer of a piece of skin from the donor site to the injured site to close the wound, is the current gold standard of wound treatment even if it creates donor-site morbidity and cannot be used in patients with extensive skin injury [2]. Tissue-engineered skin substitutes (TESS) have been developed, but dressing management can often be a slow, time-consuming process because it requires an extended cell expansion period, or in the case of allogeneic TESS that are readily available, can act only as temporary biological dressing [3]. The need for new therapeutic strategies with higher potential clinical efficacy has fostered the study of stem cells [3–5]

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