Abstract
Lipid extracts from plaques of grossly diseased human aortas were fractionated by silicic acid column chromatography. Examination of selected fractions by thin-layer chromatography revealed the presence of a number of esters with polarity intermediate between that of the triglycerides and cholesterol. Acetylation of this group of compounds decreased their polarity, thus suggesting the presence of free hydroxyl groups. Alkaline hydrolysis established that the main neutral component was cholesterol, accompanied by smaller amounts of 26-hydroxycholesterol, 7β- and 7α-hydroxycholesterol and a 24-hydroxycholesterol. The polarity of the cholesterol esters was due to their acyl moieties: three of the constituent acids have been identified as two isomeric 9-hydroxyoctadeca-10,12-dienoic acids and a 13-hydroxyoctadeca-9,11-dienoic acid. The polar sterol esters constitute a new group of lipids associated with human atheroma. The possible role of peroxidised linoleates in the formation of these compounds is discussed.
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