Abstract
Spin label electron spin resonance (ESR) techniques were used to detect changes in lipid bilayer structure caused by the addition of the envelope surface glycoprotein (G protein) of vesicular stomatitis virus (VSV) to phospholipid vesicles. G protein was extracted from intact VSV particles by solubilizing the virus envelope with lysolecithin. The extracted protein was added to sonicated egg yolk phosphatidylcholine (PC) vesicles and the detergent was removed with bovine serum albumin. The G protein to lipid ratio of the reconstituted vesicles was similar to that of the intact virus envelope and the reconstituted vesicles caused hemagglutination of the goose erythrocytes and bound to BHK cells. ESR spectra of a nitroxide-labeled stearic acid spin label incorporated into the lipid bilayer of the reconstituted vesicles indicated that the addition of G protein to PC vesicles increased the rigidity of the vesicle lipid bilayer suggesting that the hydrophobic terminus of the VSV G protein may alter the lipid bilayer structure. Addition of anti-G IgG to reconstituted vesicles caused a further increase in bilayer rigidity suggesting that cross-linking of membrane proteins may alter lipid bilayer structure.
Published Version
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