Protective efficacy of a recombinant Newcastle disease virus expressing glycoprotein of vesicular stomatitis virus in mice

Minmin Zhang,Zhiyuan Wen,Xijun Wang,Xiaofang Li,Jinying Ge,Weiye Chen,Zhigao Bu

doi:10.1186/s12985-016-0481-y

Abstract

BackgroundVesicular stomatitis virus (VSV) causes severe losses to the animal husbandry industry. In this study, a recombinant Newcastle disease virus (NDV) expressing the glycoprotein (G) of VSV (rL-VSV-G) was constructed and its pathogenicity and immune protective efficacy in mouse were evaluated.ResultsIn pathogenicity evaluation test, the analysis of the viral distribution in mouse organs and body weight change showed that rL-VSV-G was safe in mice. In immune protection assay, the recombinant rL-VSV-G triggered a high titer of neutralizing antibodies against VSV. After challenge, the wild-type (wt) VSV viral load in mouse organs was lower in rL-VSV-G group than that in rLaSota groups. wt VSV was not detected in the blood, liver, or kidneys of mice, whereas it was found in these tissues in control groups. The mice body weight had no significant change after challenge in the rL-VSV-G group. Additionally, suckling mice produced from female mice immunized with rL-VSV-G were partially protected from wt VSV challenge.ConclusionsThese results demonstrated that rL-VSV-G may be a suitable candidate vaccine against vesicular stomatitis (VS).

Highlights

Vesicular stomatitis virus (VSV) causes severe losses to the animal husbandry industry
Expression of VSV G protein by rL-VSV-G VSV Indiana strain G gene open reading frame (ORF) was inserted between P and M gene of Newcastle disease virus (NDV) genome (Fig. 1a). rL-VSV-G virus was recovered entirely from this cDNA using established reverse genetics procedures [22]
To confirm the expression of VSV G, BHK-21 cells were infected with rL-VSV-G at a multiplicity of infection (MOI) of 1

Summary

Introduction

Vesicular stomatitis virus (VSV) causes severe losses to the animal husbandry industry. In America, VSV has caused significant economic losses due to decreased milk and meat production, quarantines, trade barriers, and livestock market closures [2, 3]. This virus could spread between hoofed animals and rodents via insect vectors [4]. To induce high levels of neutralizing antibodies and protect animals from challenge by the virulent virus, the VSV antigens need to be concentrated by ultracentrifugation on sucrose gradient and be inactivated [11], which is not convenient for commercial production because it increases costs and producing-process.

Methods

Results

Conclusion