Abstract
Obesity is a multifactorial health problem resulting from genetic, environmental, and behavioral factors. A particularly interesting aspect of obesity is the differences observed in response to the same high-fat diet (HFD). In this study, we performed lipidomic profiling on livers from HFD-fed C57BL/6J mice using ultra-performance liquid chromatography–quadrupole time-of-flight mass spectrometry. Mice were divided into three groups: normal diet (ND), HFD-obesity prone (HFD-OP), and HFD-obesity resistant (HFD-OR). Principal components analyses showed a difference between the HFD-OP and HFD-OR groups. Individuals in the HFD-OR group were closer to those in the ND group compared with those in the HFD-OP group. In particular, phosphocholine (PC) and triglyceride (TG) levels differed significantly depending on the length of the acyl chain and degree of unsaturation, respectively. PC species were either positively or negatively correlated with concentrations of glucose, insulin, leptin, and hepatic cholesterol according to the length of the acyl chain. Decreased expression of the scavenger receptor B1 and ATP-binding cassette A1 in HFD-OP mice indicated that the acyl chain length of PC species may be related to high-density lipoprotein cholesterol metabolism. This study demonstrates that lipidomic profiling is an effective approach to analyzing global lipid alterations as they pertain to obesity.
Highlights
Obesity is a multifactorial health problem resulting from genetic, environmental, and behavioral factors
The average energy intake did not differ between high-fat diet (HFD) mice (Fig. 1b), whereas the feed-efficiency ratio of Plasma parameters Total cholesterol HDL cholesterol HTRa non HDL-C Apo-A1 Glucose Insulin Glucagon Leptin Triglycerides homeostasis model assessment for insulin resistance (HOMA-IR) Liver lipid content Hepatic triglycerides Hepatic cholesterol p-Value
We examined the effect of liver PC acyl chain length on the expression of lecithin-cholesterol acyltransferase (LCAT), scavenger receptor B1 (SR-B1), and ATP-binding cassette transporter A1 (ABCA1), which are related to HDL-C metabolism
Summary
Obesity is a multifactorial health problem resulting from genetic, environmental, and behavioral factors. A interesting aspect of obesity is the differences observed in response to the same high-fat diet (HFD). Decreased expression of the scavenger receptor B1 and ATP-binding cassette A1 in HFD-OP mice indicated that the acyl chain length of PC species may be related to high-density lipoprotein cholesterol metabolism. This study demonstrates that lipidomic profiling is an effective approach to analyzing global lipid alterations as they pertain to obesity. Not all individuals become obese even when consuming a high-fat diet (HFD). Recent studies have suggested that a large number of factors, including differential regulation of intestinal lipid metabolism-related genes, changes in the expression levels of liver proteins, and metabolic adaptation, are important contributors to phenotypic development[6,7,8]. Lipidomics is used to discover and identify the structures of various lipid species, examine the distribution of lipids in biofluids and tissues, and study differences between lipids in different subject groups[10,11,12]
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