Abstract
The mitochondrial lipids from avocado fruit, cauliflower buds, and potato tubers, and the lipids of chloroplasts isolated from avocado fruit and from cauliflower leaves were identified and the concentrations were determined. The lipid composition was compared with that of beef heart mitochondria. Phospholipids constituted 50-56% of total lipids in plant mitochondria while this fraction made up 90% of the lipids in beef heart mitochondria. In both cases the chief phospholipids were phosphatidylcholine and phosphatidylethanolamine. A characteristic feature of plant mitochondria was the presence of monogalactosyl- and digalactosyldiglyceride and of sulfolipid. Potato mitochondria differed from the particles of other species investigated by their higher content of galactolipids, sterol glycosides, and carotenoids and lower content of phospholipids and of total lipids in the lipidprotein complex. The galactolipid content was markedly higher in chloroplasts from all sources than in mitochondria. The spectrum of lipids in the phospholipid fraction differed more strikingly between chloroplasts of the leaf and the mitochondria of the bud of cauliflower than between the two organelles of the avocado mesocarp. The fatty acid distribution of individual lipids and of classes of lipids was also more similar in the two organelles of the fruit tissue than in the cauliflower material.
Highlights
The mitochondrial lipids from avocado fruit, cauliflower buds, and potato tubers, and the lipids of chloroplasts isolated from avocado fruit and from cauliflower leaves were identified and the concentrations were determined
Homogeneous preparations of mitochondria could be obtained without density gradient purification from potato tuber and from cauliflower buds, whereas mitochondria obtained from avocado fruit by differential centrifugation contained some chloroplast fragments and 2y0more lipid thandidthe densitygradient-purifiedparticles
The totallipidcontent of plantmitochondria was found to vary from 38y0 for avocado to 33y0 for cauliflower and 17% for Irishpotato.Phospholipids constituted 5O-56Y0 of the lipid in plant mitochondria
Summary
The mitochondrial lipids from avocado fruit, cauliflower buds, and potato tubers, and the lipids of chloroplasts isolated from avocado fruit and from cauliflower leaves were identified and the concentrations were determined. Phospholipids constituted 5&5670 of total lipids in plant mitochondria while this fraction made up 90%of the lipidsin beef heart mitochondria. In both cases the chief phospholipidws erephosphatidylcholine and phosphatidylethanolamine. The spectrum of lipids in the phospholipid fraction differed more strikingly between chloroplasts of the leaf and the mitochondria of the bud of cauliflower than between the twoorganelles of the avocadomesocarp. The lipids from plant materials were obtained mostly from tissue rather than from subcellular fractions, as exemplified by the studies on potato tuber [1] and on fruits [1,2,3,4,5]. The relatively high galactolipid content was ascribed to contamination by plastids and prompted the execution of analyticalstudiesonpurifiedsubcellularfractions by the adaptation of modern chromatographic techniques
Published Version
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