Lipid and fucoxanthin production in mixed cultures of Isochrysis galbana and Saccharomyces cerevisiae

Abstract

Event Abstract Back to Event Lipid and fucoxanthin production in mixed cultures of Isochrysis galbana and Saccharomyces cerevisiae Catarina R. Correia1*, Catarina Barraca1*, Catarina R. Mendes1, Joao Chambel1, 2 and Sergio M. Leandro1* 1 MARE – Marine and Environmental Sciences Centre, ESTM, Instituto Politécnico de Leiria, Portugal 2 AQUASPROSEA, Lda, Santuário Nossa Senhora dos Remédios, Portugal The future of aquaculture is correlated with the development, among other aspects, of the dietary elements. Feed applied in this field are in constant evolution, in order to provide a more nutritional and chemical-free solution. One of the new key-elements for the novel feeds in aquaculture is the addition of high quality lipids and natural pigments. Fucoxanthin is one of the most relevant carotenoids in brown microalgae, with several appliances, as antioxidant compound and feed addictive in aquaculture. Since lipids and this pigment have high applications in aquaculture dietary, a more economically feasible approaches for their production are in need. Pure and mixed cultures of Isochrysis galbana and Saccharomyces cerevisiae (dietary strain) were grown autotrophically in batch. Three combinations were tested: (A) Isochrysis galbana + F/2 medium, (B) Isochrysis galbana + half F/2 medium + Saccharomyces cerevisiae and (C) Isochrysis galbana + Saccharomyces cerevisiae. Cultures growth pattern was followed until second day of stationary phase was achieved, by cell manual counting and by dry weight determination. Total lipid content was performed according to modified Bligh and Dyer, total carotenoid content by spectrophotometry UV-VIS and fucoxanthin content by High Pressure Liquid Chromatography. Results attained suggest that the tested growth media did not statistically influenced biomass production, even though specific growth rate and doubling time were biologically different. Combination (A) had a µ=0.2647 d-1, (B) presented µ=0.2089 d-1 and (C) µ=0.1316 d-1. Despite having a lower specific growth-rate, combination (C) began logarithmic phase a day earlier than the other combinations tested, leading to believe that this treatment would require a fed-batch grown. Regarding total carotenoid content, combination (B) achieved higher values (1%), followed by combination (A) (0.44%) and combination (C) (0.04%). For lipid production, the combination (B) presented higher lipid productivity. This outcomes also suggest that mixed cultures of Isochrysis galbana + Saccharomyces cerevisiae, when provided with F/2 medium, are a suitable approach for lipid and fucoxanthin production. Keywords: Microalgae, Mixotrophic growth, Microalgae mass cultivation, Growth Substances, Isochrysis galbana Conference: IMMR | International Meeting on Marine Research 2016, Peniche, Portugal, 14 Jul - 15 Jul, 2016. Presentation Type: Poster presentation Topic: Blue Biotechnology Citation: Correia CR, Barraca C, Mendes CR, Chambel J and Leandro SM (2016). Lipid and fucoxanthin production in mixed cultures of Isochrysis galbana and Saccharomyces cerevisiae. Front. Mar. Sci. Conference Abstract: IMMR | International Meeting on Marine Research 2016. doi: 10.3389/conf.FMARS.2016.04.00056 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 14 May 2016; Published Online: 13 Jul 2016. * Correspondence: Dr. Catarina R Correia, MARE – Marine and Environmental Sciences Centre, ESTM, Instituto Politécnico de Leiria, 2520-641, Portugal, catarinasrcorreia@gmail.com Dr. Catarina Barraca, MARE – Marine and Environmental Sciences Centre, ESTM, Instituto Politécnico de Leiria, 2520-641, Portugal, catarinabarraca6@gmail.com PhD. Sergio M Leandro, MARE – Marine and Environmental Sciences Centre, ESTM, Instituto Politécnico de Leiria, 2520-641, Portugal, sleandro@ipleiria.pt Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Catarina R Correia Catarina Barraca Catarina R Mendes Joao Chambel Sergio M Leandro Google Catarina R Correia Catarina Barraca Catarina R Mendes Joao Chambel Sergio M Leandro Google Scholar Catarina R Correia Catarina Barraca Catarina R Mendes Joao Chambel Sergio M Leandro PubMed Catarina R Correia Catarina Barraca Catarina R Mendes Joao Chambel Sergio M Leandro Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.