Evaluation of the impact caused by handling procedures on the embryonic development of the sea urchin Paracentrotus lividus (Lamarck, 1816)

Abstract

Event Abstract Back to Event Evaluation of the impact caused by handling procedures on the embryonic development of the sea urchin Paracentrotus lividus (Lamarck, 1816) Rodolfo Ramos1*, Andreia G. Raposo1, Sílvia C. Gonçalves1, 2, Teresa M. Baptista1, José Lino V. Costa3, Ana Pombo1 and Susana M.F. Ferreira1, 4 1 Escola Superior de Turismo e Tecnologia do Mar, Politécnico de Leiria, MARE - Marine and Environmental Sciences Centre, Portugal 2 University of Coimbra, MARE – Marine and Environmental Sciences Centre, Department of Life Sciences, Faculty of Sciences and Technology, Portugal 3 Faculdade de Ciências, Universidade de Lisboa, MARE - Marine and Environmental Sciences Centre, Portugal 4 University of Coimbra, CFE - Centre for Functional Ecology, Department of Life Sciences, Portugal The international demand for sea urchin roe does urge an interest in to develop techniques to produce Paracentrotus lividus (Lamarck, 1816) in aquaculture. However, there are some obstacles that need to be overcome, like the lack of knowledge in rearing its embryos and larvae. The basic procedures to cultivate the early development stages need to be investigated and enhanced, in order to start producing sea urchin larvae in large amounts. In this study, it was evaluated the best time post-fertilisation to perform the first water exchanges and the best way to do it, based on the survival, embryonic development stage and overall water quality obtained in a laboratory assay. Several specimens were harvested from the shore of Peniche and brought to the laboratory, where they were induced to reproduce by an injection of 1 mL of potassium chloride through the peristomial membrane. Twelve cylindrical-conical recipients containing 1 L of sterile seawater were prepared with a density of 100 oocytes.mL-1. Sperm was added in a ratio of 500 spermatozoa :1 oocyte. The water was renewed 12 hours after fertilisation on 6 recipients. It was decanted on 3 of the recipients, in which the bottom layer, containing sinking unfertilised oocytes, was discarded. Afterwards, the lost volume was compensated with sterilized water. On the other hand, the embryos were filtered through an 80 µm sieve and the water entirely replaced in the other 3 recipients. The same methods were used on the 6 remaining reservoirs, but only 24 hours after fertilisation. Samples were collected during 72 hours, with a 12 hours’ periodicity. The embryos were counted and the development stage was identified. At the end of the assay, a sample of each container was collected to assess water quality through the amount of ammonia, nitrite, nitrate and phosphate. Initially, the density of P. lividus embryos was higher when water renewing occurred 12 h post-fertilisation than after 24 hours. The filtration of the embryos and larvae did not cause differential mortality, or even differences on the embryonic development, compared with decantation of the rearing water. Therefore, filtration by using a sieve is adequate and effective for P. lividus larvae’s routine processes in aquaculture, namely in the water renewal of the rearing systems, which helps maintaining a good water quality for animal welfare. Acknowledgements This project has the financial support of Operational Programme MAR2020 through the project 16-02-01-FMP-0004: Ouriceira Aqua: Aquaculture and Enhancement of Gonad Production in the Sea Urchin (Paracentrotus lividus). This study had the support of Fundação para a Ciência e Tecnologia (FCT), through the strategic project UID/MAR/04292/2013 granted to MARE - Marine and Environmental Sciences Centre. Keywords: Larvae, Aquaculture, Filtration, Decantation, Water Quality Conference: IMMR'18 | International Meeting on Marine Research 2018, Peniche, Portugal, 5 Jul - 6 Jul, 2018. Presentation Type: Poster Presentation Topic: Aquaculture Citation: Ramos R, Raposo AG, Gonçalves SC, Baptista TM, Costa JV, Pombo A and Ferreira S (2019). Evaluation of the impact caused by handling procedures on the embryonic development of the sea urchin Paracentrotus lividus (Lamarck, 1816). Front. Mar. Sci. Conference Abstract: IMMR'18 | International Meeting on Marine Research 2018. doi: 10.3389/conf.FMARS.2018.06.00025 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Apr 2018; Published Online: 07 Jan 2019. * Correspondence: PhD. Rodolfo Ramos, Escola Superior de Turismo e Tecnologia do Mar, Politécnico de Leiria, MARE - Marine and Environmental Sciences Centre, Peniche, Portugal, rodolfo-pt@hotmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Rodolfo Ramos Andreia G Raposo Sílvia C Gonçalves Teresa M Baptista José Lino V Costa Ana Pombo Susana M.F. Ferreira Google Rodolfo Ramos Andreia G Raposo Sílvia C Gonçalves Teresa M Baptista José Lino V Costa Ana Pombo Susana M.F. Ferreira Google Scholar Rodolfo Ramos Andreia G Raposo Sílvia C Gonçalves Teresa M Baptista José Lino V Costa Ana Pombo Susana M.F. Ferreira PubMed Rodolfo Ramos Andreia G Raposo Sílvia C Gonçalves Teresa M Baptista José Lino V Costa Ana Pombo Susana M.F. Ferreira Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.