Abstract
SummaryA calcifiable matrix was prepared by decalcification of marmoset femurs. Lipid extraction rendered the matrix noncalcifiable. The crude phospholipid fraction of the lipid extract induced apatite crystallinity in a metastable calcium phosphate solution. The acetone-soluble fraction did not. The results show lipid is involved in calcification of a bone matrix, in vitro, and that the nucleator is in the crude phospholipid fraction.
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