Abstract
The distribution pattern of tartrate-resistant acid phosphatase (TRAP) in matrices of developing medullary bone was histochemically examined and compared with the calcifi cation pattern of the matrix employing estrogen-induced medullary bone of male Japanesequail. Medullary bone trabeculae extended inward toward the marrow cavity and the calcification of bone matrix had begun by 4 and 7 days treatment of estrogen. The bone matrix located near the cortical bone was calcified and thin osteoid was observed. On medullary bone trabeculae more distant from cortical bone, the calcification was seen in deeper areas of the matrix with wide osteoid, and frequently the extending tips consisted solely of osteoid. Medullary bone trabeculae were more extended and the calcified areas were also increased in quails treated with estrogen for 7 days. TRAP activity was mostly positive in the matrix near the cortical bone and extending trabeculae, but surface areas and peripheries of the trabeculae were negative for the activity. The TRAP-positive area of medullary bone matrix had increased by 7 days treatment compared to that of 4 days treatment. The similarity between the distribution patterns of TRAP-positive areas in the matrix and the distribution patterns of calcified matrix suggests that the TRAP accumulated in the matrix during medullary bone formation became an active form with calcification of matrix.
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