Abstract
SummaryA method for fractionation of hog pituitary glands by saline extraction, fractional precipitation with acetone at pH 4.3, and ion-exchange chromatography, is described. The final fraction has high lipemia-producing activity in the rabbit and is free of recognized pituitary hormones. Lipemia-producing activity of this fraction is enhanced by simultaneous injection of ACTH. Production of lipemia in the rabbit by injection of ACTH together with commercial preparations of TSH, prolactin or FSH is also described. In the combination ACTH + commercial TSH, evidence is presented that ACTH acts in synergism with some “contaminating” substance in commercial TSH preparation, rather than with TSH itself.
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Schedule a callMore From: Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)
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