Experimental Studies on the Effects of Anterior Pituitary and Adrenal Cortical Hormones to the Blood Sugar and Diastase Volues of Normal and Alloxan Rabbits.

Abstract

Before and after injection of single dose of ACTH, Cortisone or DCA into normal or alloxan diabetic rabbits, the auther determined the sugar and diastase values in the blood hour by hour for 6 hours. These determinations were done repeatedly 24 hours after injections. And the auther obtained following results ; 1) In cases of normal rabbits, (a) the blood sugar values increased only after injection of Cortisone, and after injection of ACTH or DCA they intended to decrease in the beginning and then turn to approach to the values before injection. But after injection of large quantity of ACTH, the blood sugar values increased in the beginning and then decreased later. Among these 3 kinds of hormones, DCA and large quantity of ACTH showed larger blood sugar decreasing ability. (b) On the diastatic activities of the blood, they increased only after injection of large quantity of Cortisone, and after injection of Cortisone (5 mg), ACTH or DCA they intended to decrease in the beginning and then turn to approach to the value before injection. On the other hand the blood diastase values decreased hour by hour until 6 hours after injection of large quantity of DCA. Among these hormones, 5 mg Cortisone and 10 mg DCA showed stronger blood diastase decreasing abilities. (c) In the respect of the relation between curves of blood sugar and diastase values, in cases of DCA or ACTH injections they were parallel in general, but in cases of Cortisone injections they showed nearly discrepancy each other. 2) In cases of alloxan diabetic rabbits after injections of these hormones, (a) the blood sugar values varied nearly identically with the cases of normal rabbits. Also in cases of alloxan rabbits, the blood sugar increased only after injection of Cortisone, and decreased significantly by ACTH or DCA. And among these hormones, DCA had the largest blood sugar decreasing power. (b) To the diastase values of the blood, ACTH showed the most remarkable changes, and after injection of it, the blood diastatic activities showed remarkable increase. On the other hand after injection of Cortisone or DCA the sugar and diastase values of the blood decreased. (c) Concerning to the relation between the curves of blood sugar and diastase values, they showed parallel after injection of DCA, and were discrepant each other by Cortisone or ACTH.3) Comparing normal rabbits with alloxan ones on the variations of blood sugar and diastase values after injections of these three kinds of hormones, the auther found following marked differences between both kinds of rabbits. (a) After injection of ACTH there existed especially remarkable difference on blood diastase values between the both kinds of rabbits, and in cases of normal rabbits, the blood diastase values decreased slightly after the injection, but in alloxan cases they showed surprising marked increase. (b) After injection of Cortisone, the blood sugar rose in both cases, and the increasing rate in alloxan case was more slight than that of in normal case. On the other hand the blood diastase value increased moderately after injection of 10 mg Cortisone in normal case, but in alloxan case decreased and then the diastatic activity reached at the lowest level 6 hours after injection. (c) After injection of DCA, in normal case, both of the sugar and diastase values in the blood decreased slightly or moderately, but in alloxan diabetic case, both of them decreased intensively after injection.In the experimental studies above mentioned, the most impressive fact was that in cases of alloxan rabbits the blood diastatic activity increased markedly after ACTH injection, however it was decreased by Cortisone or DCA injection.The reason why the slip of the diastatic enzyme in blood is caused in case of alloxan rabbit, it is interesting theme to study.