Lipase-catalyzed enrichment of egg yolk phosphatidylcholine with conjugated linoleic acid

Abstract

The investigations were carried out to elaborate an efficient process of lipase-catalyzed acidolysis of egg yolk phosphatidylcholine (PC) with conjugated linoleic acid (CLA) taking into account both a high degree of incorporation into phospholipids (PLs) as well as the high yields of modified PLs. Therefore a term of “effective incorporation of CLA” (ECLA) was proposed as the most complex criterion for the evaluation of effectiveness of the process. The best results were achieved when Lipozyme RM IM was applied and the reaction conditions were as follows: CLA/PC molar ratio 8:1; enzyme loading 24% wt. based on substrates; reaction medium heptane; temperature 45°C; time 36h. Reaction was conducted under initial aw=0.33 which was changed after 12h to aw=0.11. Under these conditions incorporation of CLA (calculated as % of total FA) into modified PC and lysophosphatidylcholine (LPC) reached 33.8% and 50.1%, respectively. The ECLA incorporation reached 39.0%. The yields of PC and LPC were 39.5% and 25.3%, respectively. The results showed the highest effective incorporation of CLA into PC in comparison to the data reported previously.