LIPASE PRODUCTION BY Aspergillus niger GROWN IN DIFFERENT AGRO-INDUSTRIAL WASTES BY SOLID-STATE FERMENTATION

Tania Maria Costa,Miguel Garcia-Roman,Katia Luiza Hermann,Lorena Benathar Ballod Tavares,Rita De Cassia Siqueira Curto Valle

doi:10.1590/0104-6632.20170342s20150477

Tania Maria Costa, Miguel Garcia-Roman + Show 3 more

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https://doi.org/10.1590/0104-6632.20170342s20150477

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Abstract

Abstract – Filamentous fungi can easily degrade agro-industrial wastes in solid-state fermentation processes, synthesizing many important commercial biocompounds, such as lipolytic enzymes. The aim of this study was to evaluate the effect of the composition of the solid culture medium on the production of lipolytic enzymes by the fungus Aspergillus niger. Rice, wheat and soybean bran were mixed to prepare the culture medium, which was supplemented with glucose, glycerol or soybean oil. Four mixture experimental designs were used to find the best medium for enzyme production. According to our results, the highest lipolytic activity values were achieved with a mixture of rice bran and glycerol (19.844 U·g -1 ) or with rice bran only (13.267 U·g). Thus, the lipolytic enzyme could be produced without any additional carbon source apart from rice bran, although glycerol addition induced a higher production. Keywords : Enzyme; Fungus; Agro-industrial wastes; Carbon source. INTRODUCTION Lipases (triacylglycerol acyl hydrolases, EC 3.1.1.3) are enzymes that catalyze the hydrolysis and the synthesis of esters (Qamsari et al., 2011). Some lipases are capable of catalyzing the esterification, transesterification and interesterification of lipids (Burkert et al., 2004; Colla et al., 2010; Martins et al., 2008), water activity (a

Highlights

Lipases are enzymes that catalyze the hydrolysis and the synthesis of esters (Qamsari et al, 2011)
It only needs a low percentage of free water to be conducted and it uses low cost substrates such as agricultural wastes, which act both as a source of nutrients for the fermentation process (Martins et al, 2008; Messias et al, 2011; Pandey, 2003)
Agro-industrial wastes have been used as carbon source for microbial growth and lipase production by Aspergillus niger, a fungus which belongs to a prominent and important genera of filamentous ascomycetes

Summary

Introduction

Lipases (triacylglycerol acyl hydrolases, EC 3.1.1.3) are enzymes that catalyze the hydrolysis and the synthesis of esters (Qamsari et al, 2011). Solid-state fermentation studies involving different wastes and microorganisms have achieved good results with low enzyme production costs (Colla et al, 2010; Martins et al, 2008) Among these microorganisms, filamentous fungi are considered the most suitable for processes involving SSF due to their ability to grow with a low amount of free water (Castiglioni et al, 2009; Thomas et al, 2013; Vendruscolo et al, 2007) together with their efficiency to degrade some pollutants (Rigas et al, 2007; Ye et al, 2011). The production of lipolytic enzymes by filamentous fungi in SSF from agro-industrial wastes has attracted great interest, since these enzymes can be extracted from the fermentation medium as they are mostly extracellular (Moura et al, 2013; Roveda et al, 2010)

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