Abstract
BackgroundTongue squamous cell carcinoma (TSCC) is one of the most common oral tumors. Recently, long intergenic noncoding RNA 00958 (LINC00958) has been identified as an oncogene in human cancers. Nevertheless, the role of LINC00958 and its downstream mechanisms in TSCC is still unknown.MethodsThe effect of LINC00958 on TSCC cells proliferation and growth were assessed by CCK-8, colony formation, 5-Ethynyl-2′-deoxyuridline (EdU) assay and flow cytometry assays in vitro and tumor xenograft model in vivo. Bioinformatics analysis was used to predict the target of LINC00958 in TSCC, which was verified by RNA immunoprecipitation and luciferase reporter assays.ResultsLINC00958 was increased in TSCC tissues, and patients with high LINC00958 expression had a shorter overall survival. LINC00958 knockdown significantly decreased the growth rate of TSCC cells both in vitro and in vivo. In mechanism, LINC00958 acted as a ceRNA by competitively sponging miR-211-5p. In addition, we identified CENPK as a direct target gene of miR-211-5p, which was higher in TSCC tissues than that in adjacent normal tissues. Up-regulated miR-211-5p or down-regulated CENPK could abolish LINC00958-induced proliferation promotion in TSCC cells. Furthermore, The overexpression of CENPK promoted the expression of oncogenic cell cycle regulators and activated the JAK/STAT3 signaling.ConclusionsOur findings suggested that LINC00958 is a potential prognostic biomarker in TSCC.
Highlights
Tongue squamous cell carcinoma is one of the most frequently diagnosed malignancies in the oral cavity [1, 2]
LncRNAs in Tongue squamous cell carcinoma (TSCC) tissues from different patients at different stages were screened based on the Cancer Genome Atlas (TCGA) database analysis, and we identified that LINC00958 was the most up-regulated Long non-coding RNAs (lncRNAs) in the TSCC tissues
LINC00958 is up‐regulated in tongue cancer tissues and cell lines Using TCGA database analysis, we detected aberrantly expressed lncRNAs between adjacent normal tissues and TSCC tissues from TSCC patients
Summary
Tongue squamous cell carcinoma is one of the most frequently diagnosed malignancies in the oral cavity [1, 2]. Because of its high metastatic and proliferative ability, TSCC is a considerable threat to human health worldwide [3, 4]. Increasing evidence has proved that Long non-coding RNAs (lncRNAs) modulate diverse physiologic and pathologic processes, including proliferation, Jia et al Cancer Cell Int (2021) 21:147 migration, invasion, and autophagy[7]. It has been reported as a core regulator in the processes of chromatin modification, transcriptional regulation, alternative splicing and interaction with RNAs and protein complex at transcriptional or posttranscriptional levels[8]. The role of LINC00958 and its downstream mechanisms in TSCC is still unknown
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