Abstract
BackgroundCervical cancer (CC) is one of the most common malignancies affecting female worldwide. Long non-coding RNAs (lncRNAs) are increasingly indicated as crucial participants and promising therapeutic targets in human cancers. The main objective of this study was to explore the functions and mechanism of LINC00885 in CC.MethodsRT-qPCR and western blot were used to detect RNA and protein levels. Functional and mechanism assays were respectively done for the analysis of cell behaviors and molecular interplays.ResultsLong intergenic non-coding RNA 885 (LINC00885) was discovered to be upregulated in CC tissues and cell lines through bioinformatics analysis and RT-qPCR. Overexpression of LINC00885 promoted proliferation and inhibited apoptosis, whereas its silence exerted opposite effects. The cytoplasmic localization of LINC00885 was ascertained and furthermore, LINC00885 competitively bound with miR-3150b-3p to upregulate BAZ2A expression in CC cells. Rescue assays confirmed that LINC00885 regulated CC proliferation and apoptosis through miR-3150b-3p/BAZ2A axis. Finally, we confirmed that LINC00885 aggravated tumor growth through animal experiments.ConclusionsLINC00885 exerted oncogenic function in CC via regulating miR-3150b-3p/BAZ2A axis. These findings suggested LINC00885 might serve as a potential promising therapeutic target for CC patients.
Highlights
Cervical cancer (CC) is one of the most common malignancies affecting female worldwide
LINC00885 was upregulated in CC and predicted poor prognosis To explore the functions of LINC00885 in CC, the expression pattern of LINC00885 in cervical squamous cell carcinoma (CESC) samples was firstly obtained from GEPIA database, a comprehensive resource for systematic analysis of gene expression [31]
It was found that LINC00885 presented significantly higher level in CESC samples (Fig. 1a), indicating that LINC00885 might be associated with CC progression
Summary
Cervical cancer (CC) is one of the most common malignancies affecting female worldwide. Long noncoding RNAs (lncRNAs) are increasingly indicated as crucial participants and promising therapeutic targets in human cancers. The main objective of this study was to explore the functions and mechanism of LINC00885 in CC. Cervical cancer (CC), one of leading causes of cancer death, has approximately 527,600 cases of diagnosis and 265,700 cases of death globally in 2012 [1]. The dysregulation and function of lncRNAs have been revealed in numerous cancers, indicating that lncRNAs could be promising prognostic biomarkers in cancers [12,13,14]. Many lncRNAs have been proven to exert regulatory functions in cervical cancer [15, 16]. Present study identified long intergenic non-coding RNA 885 (LINC00885) to be upregulated in cervical cancer.
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