Abstract
BackgroundDysregulation of long non-coding RNAs (lncRNAs) has been identified in ovarian cancer. However, the expression and biological functions of LINC00852 in ovarian cancer are not understood.MethodsThe expressions of LINC00852, miR-140-3p and AGTR1 mRNA in ovarian cancer tissues and cells were detected by quantitative reverse transcription polymerase chain reaction (qRT-PCR) assay. Gain- and loss-of-function assays were performed to explore the biological functions of LINC00852 and miR-140-3p in the progression of ovarian cancer in vitro. The bindings between LINC00852 and miR-140-3p were confirmed by luciferase reporter gene assay, RNA immunoprecipitation (RIP) assay and RNA pull-down assay.ResultsWe found that LINC00852 expression was significantly up-regulated in ovarian cancer tissues and cells, whereas miR-140-3p expression was significantly down-regulated in ovarian cancer tissues. Functionally, LINC00852 knockdown inhibited the viability, proliferation and invasion of ovarian cancer cells, and promoted the apoptosis of ovarian cancer cells. Further investigation showed that LINC00852 interacted with miR-140-3p, and miR-140-3p overexpression suppressed the viability, proliferation and invasion of ovarian cancer cells. In addition, miR-140-3p interacted with AGTR1 and negatively regulated its level in ovarian cancer cells. Mechanistically, we found that LINC00852 acted as a ceRNA of miR-140-3p to promote AGTR1 expression and activate MEK/ERK/STAT3 pathway. Finally, LINC00852 knockdown inhibited the growth and invasion ovarian cancer in vivo.ConclusionLINC00852/miR-140-3p/AGTR1 is an important pathway to promote the proliferation and invasion of ovarian cancer.
Highlights
Dysregulation of long non-coding RNAs has been identified in ovarian cancer
LINC00852 is highly expressed in ovarian cancer As shown in Fig. 1a, LINC00852 expression was significantly up-regulated in ovarian cancer tissues than adjacent normal tissues
After SKOV-3 and OV-90 cells transfected with sh-LINC00852-1, shLINC00852-2 and sh-LINC00852-3, LINC00852 expression was significantly down-regulated in SKOV-3 and OV-90 cells (Fig. 2a), and results showed that shLINC00852-1 obtained the best transfection efficiency (Fig. 2a)
Summary
Dysregulation of long non-coding RNAs (lncRNAs) has been identified in ovarian cancer. The expression and biological functions of LINC00852 in ovarian cancer are not understood. Ovarian cancer is usually diagnosed at advanced stages due to the lack of typical clinical symptom and effective clinical diagnosis method at early stages [2, 3]. It has been found that the development of ovarian cancer is closely related with the proliferation, invasion and migration of ovarian cancer cells [4]. The metastasis of ovarian cancer usually leads to the recurrence and poor prognosis of ovarian cancer [5]. The metastasis and recurrence are needed to be controlled to improve the prognosis
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