LINC00205 promotes malignancy in lung cancer by recruiting FUS and stabilizing CSDE1.

Peng Xie,Yesong Guo

doi:10.1042/bsr20190701

Abstract

Lung cancer (LC) is characterized by high morbidity and mortality. Numerous long noncoding RNAs (lncRNAs) have been reported to be involved in the initiation and progression of human cancers, including LC. Long intergenic non-protein coding RNA 205 (LINC00205) is identified as a novel lncRNA, which has only been unmasked to be a potential cancer promoter in hepatocellular carcinoma and pancreatic cancer. The biologic function and the molecular mechanism of LINC00205 in LC require to be investigated. In the present study, we observed the elevated expression of LINC00205 in LC tissues and cells through real-time quantitative PCR (RT-qPCR). Additionally, silencing LINC00205 inhibited LC cell growth and migration, but aggravated cell apoptosis. Moreover, we found that LINC00205 recruited FUS to maintain the mRNA stability of cold shock domain containing E1 (CSDE1) and therefore up-regulated CSDE1 expression in LC. Further, the effects of LINC00205 on LC cell proliferation, apoptosis and migration were all erased by CSDE1 overexpression. These findings demonstrated that LINC00205 facilitates malignant phenotypes in LC by recruiting FUS to stabilize CSDE1, suggesting LINC00205 as a potential target for LC therapy.

Highlights

Lung cancer (LC) has been characterized as a malignancy with the highest mortality, with the 5-year survival rate of approximately 20% [1,2]
The results of real-time quantitative PCR (RT-qPCR) elucidated that LC tissues expressed higher level of LINC00205 than paired non-tumor tissues (Supplementary Figure S1A)
Flow cytometry analysis indicated that LINC00205 depletion resulted in accelerated apoptosis in LC cells (Supplementary Figure S1B)

Summary

Introduction

Lung cancer (LC) has been characterized as a malignancy with the highest mortality, with the 5-year survival rate of approximately 20% [1,2]. Long noncoding RNAs (lncRNAs) are featured with none protein coding capacity and the length of more than 200 nucleotides [5,6]. Wang et al found that lncRNA EGFR-AS1 promotes cell growth and metastasis in renal cancer [7]. Shi et al discovered that lncRNA ZNFX1-AS1 facilitates tumor progression and metastasis in colorectal cancer [8]. Shi et al revealed lncRNA FAM83A-AS1 as a tumor promoter in LC [9]. Long intergenic non-protein coding RNA 205 (LINC00205) is located at chromosome 21, NC 000021.9, and it is named as LOC642852. LINC00205 is an lncRNA with poor annotation. Cui et al supported that LINC00205 overexpression contributes to poor prognosis of patients with hepatocellular cancer [10]. The role of LINC00205 in LC is still veiled

Methods

Results

Conclusion